EPR of heme proteins in mesoporous matrices – a successful match between spectroscopy, protein research, and hybrid material and biosensor development

Main list: Physics and Astronomy Colloquia

Abstract

Prof. Sabine Van Doorslaer
Antwerp University

The incorporation of proteins in mesoporous materials provides a promising tool to create new hybrid materials for biosensing or biocatalysis applications. The confinement of the proteins in these materials may stabilize the protein structure and even improve protein activity, but finding the optimal incorporation conditions and best protein-matrix matches is not trivial. This requires not only an in-depth understanding of the biochemical working of the used proteins under diverse chemical conditions and knowledge of the characteristics of the mesoporous host, it also calls for a variety of characterization tools that can probe the individual components as well as the hybrid materials. Although simple optical absorption techniques can be used to follow the adsorption and incorporation of the proteins, the faith of the proteins inside the synthetic host needs to be addressed by different techniques. Using the example of the incorporation of heme proteins, more specifically globins and peroxidases, in mesoporous titania and silica, it will be shown that EPR can provide unique information that cannot be obtained by other characterization methods. Moreover, EPR can play a role in monitoring and understand radical-formation reactions during the working of the final electrochemical biosensor. This requires developments of improved in-situ EPR hydrodynamic electrochemical cells, allowing fast and easy replacement of parts and adjustments to electrodes.

  • Venue

    Physics THeatre C

  • Date

    November 15, 2019

  • Time

    From: 10h00 To: 11h00

  • Sponsor

    University of St Andrews
    The oldest university in Scotland, with international renown for both research and education of undergraduates and postgraduates.

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