Advancing metalloprotein design for new functions and applications

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Abstract

Dr Anna Peacock
Birmingham
Advancing metalloprotein design for new functions and applications Anna F. A. Peacock School of Chemistry, University of Birmingham, Edgbaston, B15 2TT, UK. a.f.a.peacock@bham.ac.uk De novo designed miniatur e protein scaffolds, such as the coiled coil, offer exciting opportunities for metal ion coordination. [1] Not surprisingly due to the protein like nature of the scaffold, the large majority of de novo metallocoiled coil examples have focused their efforts on mimicking the active sites of native metalloenzymes. Our approach is to instead to use these artificial proteins as novel ligands for the coordination of xeno metals, with no known biological role, with the view to developing functional systems for val uable applications beyond the scope of nature. We recently reported the design of the first gadolinium coiled coil, which displayed promise as a potential MRI contrast agent. [2,3] We have since interrogated the coordination of various lanthanide ions to o ur coiled coils and have for the first time shown that we can selectively discriminate between Ln(III) ions based on size. As a result we have now designed coiled coils capable of binding two different Ln(III) ions selectively to two different sites (see F igure), and at a defined distance from one another. The opportunities this affords and the potential applications of this new class of lanthanide coiled coils, will be discussed. Generous financial support from The University of Birmingham, the School of Chemistry and EU COST action CM1105 is acknowledged.

References [1] A. F. A. Peacock, Cur. Opin. Chem. Biol. 17 (2013) 934 -939. [2] M. R. Berwick et al. , J. Am. Chem. Soc. 136 (2014) 1166 -1169. [3] M. R. Berwick et al. , Chem. Sci. 7 (2016) 2207 -2216.
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  • Venue

    Theatre C, Purdie

  • Date

    October 2, 2019

  • Time

    From: 15h30 To: 16h30

  • Sponsor

    University of St Andrews
    The oldest university in Scotland, with international renown for both research and education of undergraduates and postgraduates.

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